The microscope enclosure creates a large volume around the microscope in which temperature is controlled by recirculating warm air. It is made of polycarbonate panels and encloses microscope stage, nosepiece and objectives. It doesn't affect focus stability. All electronics including EMCCDs, spinning disk confocal etc. are left outside of the enclosure avoiding exposure to 37°C.
The enclosure closely follows the microscope profile and is supported by posts resting on the vibration isolation table.
The H201-LG-DLS Gas Chamber connects to Bold Line Temperature, Gas and Humidity Controllers and allows to perform time lapse imaging using the Leica Digital Light Sheet on living samples by controlling Temperature, Humidity and Gas concentration.
The Gas Chamber fits into the Super Z Galvo stage and features a lid to accommodate the TwinFlect mirror device. The warm air circulating in the enclosure warms up the detection objectives Leica HC APO L 10x/0.3 W and Leica HC FLUOTAR L 25x/0.95 W.
Compatible with Leica DLS module, it allows usage of illumination and detection objectives. Fits in the Super Z galvo stage, by replacing the Leica specimen holder
The chamber can fit:
35/60 mm Petri dishes
Compatible with Leica DLS module, it allows usage of illumination and detection objectives
Includes an objective collar for detection objectives Leica HC APO L 10x/0.3 W and Leica HC FLUOTAR L 25x/0.95 W
Uniformly heated by means of embedded electric resistances and covered with a temperature controlled lid
Spring locks hold Petri dishes, in correct position inside the chamber
Perfusion holes: 12 channels for 2.0 mm O.D. tubings are available
The chamber screws onto Super Z galvo stage
Chamber weight, 130 g.
To control the humidity into the chamber humidity module is recommended: HM-ACTIVE
#1 35mm Petri-dish holder
#1 60mm Petri-dish holder
Okolab Cage Incubator employs a Temperature Unit that generates warm air to raise the temperature inside the enclosure.
Superior accuracy is guaranteed by the interplay of four sensors: three for temperature and one for flow rate. The temperature sensors monitor temperature inside the enclosure, inside the Gas Micro-Environmental Chamber and inside the Air Heater. The flow rate sensor controls the rate at which warm air enters the enclosure and ensures smooth operation and fast heating. A proprietary smart algorithm determines the minimum flow rate necessary to maintain the controlled temperature at the set point value.
Okolab Cage Incubation System has both Enclosure (Chamber) Feedback mode as well as Sample Feedback mode as standard features. The end user can select the temperature sensor driving feedback to the controller.
Temperature range: from 3°C above ambient to 45°C
Temperature accuracy: 0.1°C
# 3 Temperature sensors: in the enclosure; in the gas chamber; inside the air heater
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
CO2 - O2 CONTROLLERS
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
A vibration free humidity module is typically used with a Cage Incubation System. When Humidity control with feedback is required, the Active Humidity Controller can be connected to the Bold Line Gas Controller.
CO2 accuracy: 0.1% (see Controller page for more info)
O2 accuracy: 0.1% (see Controller page for more info)
Touch Screen Interface - OKO-TOUCH
Output Flow Rate: adjustable from OKO-TOUCH
Compatible with Active Humidity Controller
Programmable CO2 (and /or) O2 cycles
Compatible with SMART BOX for data logging and remote operation - Optional
Data Logging via OKOLAB Data Log Software - Optional
Compatible with LAS X
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Hypoxia and / or Hyperoxia ?
Oxygen control is our pride.
Check out the available solutions in the Controller List
Multiple units ? Be Smart - go central
One controller feeds up to 10 microscope incubators.
The Cage Incubator is equipped with a vibration-free gas humidifier which employes a semi-permeable membrane. Humidification occurs without bubbling to ensure vibration-free operation.
A water trap is located between the humidifier and the gas chamber preventing any water droplet from accidentally reaching the sample.
Gas is humidified through a semi-permeable membrane
No bubbling, i.e. no vibrations
Fits inside the enclosure
Re-filler without opening the enclosure and without perturbing the sample
If active control of humidity is needed, see HM-ACTIVE
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
It allows to:
Turn all Bold Line Controllers on/off
Define set point values
Set flow rates of the Temperature Unit
Set flow rates gas controller
Define gas cycles of gas controller
Select sample or enclosure feedback mode
Display all set points and measured parameters
Run self calibration procedures
Smart Box connects to the Bold Line Controller and provides the following features:
Remote Operation of the incubator via Internets, Tablet or SmartPhone